Primer coverage

LSU区域的引物覆盖情况

This page lists an overview of the coverage and additional information for LSU primers and primer pairs. The coverage information can be viewed either as “% in Species” or “% in Sequences”. Specific terms are defined as follows:

Length = The primer length, measured in base pairs
Coverage % in Sequences = The percent of sequences covered within this taxon, considering four conditions: (1) all three domains (All), (2) Bacteria only (Bacterial %), (3) Archaea only (Archaeal %), or (4) Eukaryota only (Eukaryotic %). It’s calculated with the equation {number of sequences within this taxon that can be amplified using the specified primer pair} / {total number of sequences in the database* from this taxa}
Coverage % in Species = The percent of species covered within this taxon, considering four conditions: (1) all three domains (All), (2) Bacteria only (Bacterial %), (3) Archaea only (Archaeal %), or (4) Eukaryota only (Eukaryotic %). It’s calculated with the equation {number of species within this taxon that produce at least one amplicon using the specified primer pair} / {total number of species in the database* from this taxa}
Amplicon Length =
Melting Temperature =

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DSPN name	Long name	Sequence	Length	All%	Bacterial%	Archaeal%	Eukaryotic%	Amplicon Length	Melting temperature	Dimer formation	GC content
LU455ar	L-*-Univ-0455-a-A-15	TTCCCTCACGGTACT	15	0.681971	0.561728	0.816587	0.950368	-	39	39	53.33
LU455af-LU2585ar	L--Univ-0455-a-S-15 L--Univ-2585-a-A-18	AGTACCGTGAGGGAA TCCCGAAGTTACGTTCT	15 18	0.00210392	0.00294118	0	0.00030774	1206.76082474227	39 43	39 43	53.33 47.06
LU455af-LU2496ar	L--Univ-0455-a-S-15 L--Univ-2496-a-S-15	AGTACCGTGAGGGAA GAGCCGACATCGAGG	15 15	0.741676	0.881049	0.280247	0.449608	2103.20936036752	39 43	39 43	53.33 66.67
LU455af-LU1683ar	L--Univ-0455-a-S-15 L--Univ-1683-a-A-19	AGTACCGTGAGGGAA CCTTCTCCCGAAGTTACGG	15 19	0.703302	0.786798	0.350617	0.533082	1291.11718554568	39 53	39 53	53.33 57.89
LU455af-LE1246ar	L-D-Euka-1246-a-A-27 L-*-Univ-0455-a-S-15	ATTTGCTACTACCACCAAGATCTGCAC AGTACCGTGAGGGAA	27 15	0.207784	0.000167112	0	0.698646	1193.63927701577	71 39	71 39	44.44 53.33
LU455af-LE1124ar	L-D-Euka-1124-a-A-15 L-*-Univ-0455-a-S-15	AGCGCCATCCATTTT AGTACCGTGAGGGAA	15 15	0.22361	0.00167112	0.123457	0.740729	1055.89919331096	37 39	37 39	46.67 53.33
LU455af-LE1077ar	L-D-Euka-1077-a-A-27 L-*-Univ-0455-a-S-15	GGTGAGTTGTTACACACTCCTTAGCGG AGTACCGTGAGGGAA	27 15	0.222055	0.000167112	0	0.746653	1015.04416517056	75 39	75 39	51.85 53.33
LU455af-LE0949ar	L-D-Euka-0949-a-A-21 L-*-Univ-0455-a-S-15	CGCCAGTTCTGCTTACCAAAA AGTACCGTGAGGGAA	21 15	0.216886	0.000200535	0	0.729189	885.011379541167	55 39	55 39	47.62 53.33
LU455af-LB2742ar	L-D-Bact-2742-a-A-16 L-*-Univ-0455-a-S-15	CTTAGATGCTTTCAGC AGTACCGTGAGGGAA	16 15	0.5861	0.833556	0.4	0.0280812	2281.35042251347	39 39	39 39	43.75 53.33
LU455af-LB2651ar	L-D-Bact-2651-a-A-15 L-*-Univ-0455-a-S-15	GGTCCTCTCGTACTA AGTACCGTGAGGGAA	15 15	0.65091	0.858924	0.202469	0.200031	2227.42142966731	39 39	39 39	53.33 53.33